FastQCFastQC Report
Wed 25 May 2016
SRR1700169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8658362
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112520.12995529639439884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA182500.022.135911
GGTATCA91800.021.7458671
ATCAACG225700.017.573513
TCAACGC225700.017.5630344
CAACGCA230000.017.2636285
TATCAAC236200.016.916612
AACGCAG237200.016.8155756
TATAACG5300.015.2818852
ACGCAGA272050.014.6199617
CGCAGAG278200.014.26442058
ATAACGC6000.014.2485323
AGAGTAC269750.013.98570311
GAGTACT171950.013.658751512
GTATTAG23750.013.45614051
GTACATG217850.013.2028921
AGTACTT179000.013.04531313
GTACCTG41600.012.9841571
GCAGAGT305750.012.8908149
CAGAGTA300150.012.88398410
ACCGCGT3001.2914825E-1012.7479518
Produced by FastQC (version 0.11.5)