FastQCFastQC Report
Wed 25 May 2016
SRR1700168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5557364
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89090.16030981594871235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61550.11075394737505048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64750.026.2753871
GTACCTG45700.026.0991761
TACCTGG46750.025.3612732
TATAACG4200.022.4979062
ACCTGGG52850.021.795513
GTATCAA126900.021.6886221
ATAACGC5200.020.7672963
TAACGCA5200.018.6035334
ATCAACG163600.016.570893
CCTGGGG44350.016.4354534
TCAACGC164700.016.4324514
CAACGCA167250.016.1814755
TATCAAC168800.016.1403792
AACGCAG174600.015.6033746
TACCGCG2850.014.9977928
CGCGGTA2052.1860615E-814.26696343
TCACGCA5550.014.1874834
TAGACCG1301.9306304E-413.8441165
ACGCAGA200500.013.5539947
CGCAGAG202700.013.4070058
Produced by FastQC (version 0.11.5)