FastQCFastQC Report
Wed 25 May 2016
SRR1700167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7024120
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88100.12542496426598634No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA78210.11134490868607028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA180550.018.3508991
GGTATCA108000.016.3186781
TCAACGC215100.015.0611724
ATCAACG215600.015.0372143
CAACGCA217700.014.8805555
AACGCAG219750.014.7622116
TATCAAC224500.014.5718822
CGCGGTA3500.014.14116343
GTACATG176400.014.1252461
GTATTAG20950.013.9671011
AGAGTAC214350.013.9058711
GAGTACT129500.013.879786512
TACATGG178550.013.5083572
TATAACG5200.013.4132
AGTACTT133500.013.295403513
ACGCAGA250050.012.9281297
GTATAAT25600.012.9248151
ACATGGG179500.012.9099613
CGCGTAC1952.7695678E-612.69006410
CGCAGAG254100.012.6423948
Produced by FastQC (version 0.11.5)