FastQCFastQC Report
Wed 25 May 2016
SRR1700166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6572587
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103100.1568636520140395No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA75620.11505363108924994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70260.10689854694962578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG50150.022.4374141
TAACGCA4150.022.2263134
ATAACGC5000.021.1478443
TACCTGG52500.021.0837252
ACCTGGG54600.019.8194083
TATAACG5100.019.4099332
GTATCAA187050.017.9628731
GGTATCA113350.016.6179581
GTACCGG5100.015.003151
CCTGGGG42250.014.6965034
ATCAACG224050.014.6001633
TCAACGC226350.014.44164854
CGCGGTA2651.6370905E-1114.43249143
CAACGCA231150.014.1997215
TATCAAC231800.014.1897742
AACGCAG236250.013.9885186
GTATTAG20000.013.9529291
ACAACGC3009.094947E-1213.4986243
GTACATG176100.013.4823881
TACACGG4850.013.4523392
Produced by FastQC (version 0.11.5)