FastQCFastQC Report
Wed 25 May 2016
SRR1700165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8643238
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131490.15213048628303422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96030.11110419497877995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87150.029.3368821
GTATCAA184800.023.5170781
ATCAACG238250.017.8944573
TCAACGC238800.017.8618374
CAACGCA242400.017.6054345
TATCAAC248600.017.3309522
AACGCAG249950.017.1636496
ACGCAGA291000.014.7347227
CGCAGAG294750.014.5090968
ATAACGC4800.013.5924883
GAGTACT196350.013.46229312
AGAGTAC308250.013.41388111
GCAGAGT322350.013.21795759
CAGAGTA318600.013.14052210
TATACCG3650.012.9431065
AGTACTT207700.012.75912413
TAACGCA5000.012.5982484
TCTATAC23300.012.3593673
GTACATG222500.012.3405031
GTACTTT217750.012.04626614
Produced by FastQC (version 0.11.5)