FastQCFastQC Report
Wed 25 May 2016
SRR1700164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6241526
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66110.10591961004408218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62950.10085674560996781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101650.020.1933291
GTATCAA161050.015.8233651
ACGAACG2002.5007466E-713.49788529
TATACTG26200.012.8789395
CGAACGC2653.5561243E-912.73385430
ATCAACG203150.012.3573143
TCAACGC205450.012.1860994
TACCGCG3152.910383E-1012.1403718
TATCAAC209200.012.0647112
CAACGCA207700.012.0544885
TCGCCCC3753.6379788E-1211.99754238
TTAGCGA2851.0484655E-811.8400627
AACGCAG212200.011.8094566
TATACAG28400.011.643655
ACGTCCG2901.3566023E-811.63573543
TAGACCG1558.5167645E-411.6104335
AACGCAC2758.114148E-811.45293532
TACACTG24350.011.3630865
ATATACT35150.011.2093064
ATAACGC4253.6379788E-1211.12490753
Produced by FastQC (version 0.11.5)