FastQCFastQC Report
Wed 25 May 2016
SRR1700163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11996808
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA285230.2377549094725864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132990.11085448729362012No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA127790.1065200009869292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA399950.014.8722251
AGAGTAC384050.013.371881511
GAGTACT226300.013.10102512
GTACATG306100.012.8344221
AGTACTT236200.012.46609713
ATCAACG471550.012.3826273
TCAACGC471900.012.3734434
TACATGG304200.012.3032732
CAACGCA475600.012.2734395
AACGCAG478300.012.223076
GGTATCA273400.012.2177711
TATCAAC485450.012.0840382
TCTATAC31900.012.0710063
CGCGGTA4500.011.99746143
GTACTTT245700.011.89254414
TAGACCG3052.2700988E-911.8002425
TACCGCG5400.011.6637618
ACATGGG306850.011.6609983
GTATTAG35950.011.3480681
GTATATA62350.011.3148851
Produced by FastQC (version 0.11.5)