FastQCFastQC Report
Wed 25 May 2016
SRR1700162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4819849
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA105290.2184508269864886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56090.11637294031410528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53820.1116632492013754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG35600.020.8223921
TACCTGG35000.020.5869242
TATAACG2950.019.0821572
ACCTGGG38000.018.1911333
TAACGCA3400.016.5564064
ATAACGC3600.016.262073
GGTATCA122100.014.55948451
CCTGGGG32700.013.4273984
GTACACG3101.6370905E-1113.0826431
GTATCAA166850.012.7341561
ACGAACG1250.002255338512.598529
TATATCG2156.024129E-712.5553445
ATAATAC20450.012.5521043
GTGTAAG12100.012.4759641
GTACCGG3900.012.13219451
TAATCCG1701.3823799E-411.9091125
CGTATCG1350.00401693611.66406915
TATACTG19000.011.6026795
CGCGTGA1400.0050987411.29718745
TACACGG3602.2009772E-1011.2584732
Produced by FastQC (version 0.11.5)