FastQCFastQC Report
Wed 25 May 2016
SRR1700161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10966317
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132650.12096130359901142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114800.025.3329181
GTATCAA223700.022.1714481
ATCAACG277700.017.3876933
TCAACGC278250.017.3047624
CAACGCA281500.017.0919845
TATCAAC288650.016.9000452
AACGCAG290050.016.6503436
ACGCAGA339200.014.1912937
CGCAGAG344350.013.9529248
GTATAGG18550.013.8547371
AGAGTAC341850.013.37063911
GTACCTG57100.013.3449811
GAGTACT190500.013.28377712
TATACTG40200.013.0933245
GTACATG296350.013.0313631
TATAACG6150.012.8172232
CAGAGTA368950.012.74215410
GCAGAGT378300.012.653179
TACCTGG57900.012.4861192
AGTACTT200000.012.472733513
Produced by FastQC (version 0.11.5)