FastQCFastQC Report
Wed 25 May 2016
SRR1700160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6184489
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88250.019.969491
GTATCAA132400.015.3870511
CGTATAC3000.014.2606113
CGACGTT1555.830186E-513.06155814
TAATACT24900.012.3887354
TAGACTG12900.012.3809185
ATAATAC24950.012.2736613
TACACTG25950.011.7025535
TCAACGC169900.011.6890964
ATCAACG170300.011.6616423
TATACTG24000.011.528645
GTATACG2351.7257371E-611.5076931
CAACGCA172600.011.4820475
GTAATAC16550.011.4284423
TATCAAC175850.011.4219112
TATACAG27500.011.2883535
TACCGCG2201.0640837E-511.2474538
AACGCAG177600.011.20945556
GTAGCAC9050.011.1961753
CCGTTAA2851.3317731E-711.0501316
Produced by FastQC (version 0.11.5)