FastQCFastQC Report
Wed 25 May 2016
SRR1700159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5893983
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85550.14514802638555285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68420.11608448819753975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59550.029.0638391
GTACCTG48450.025.34991
TACCTGG47800.025.0160732
GTATCAA121200.022.1081681
TATAACG4850.021.8227062
ATAACGC5200.021.2198143
ACCTGGG55900.020.9881783
TAACGCA5550.020.2873734
ATCAACG157250.016.4112913
TCAACGC159100.016.2629244
CAACGCA162250.016.040655
TATCAAC164300.015.8305832
GTACACG4600.015.6770971
AACGCAG170100.015.4592076
TATCACG5150.015.3042942
TCACGCA5300.015.2959164
CCTGGGG45600.015.2595764
GTACCGG4350.014.5058211
TACACGG4450.014.1693692
TATACTG21000.013.7108375
Produced by FastQC (version 0.11.5)