FastQCFastQC Report
Wed 25 May 2016
SRR1700158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10006438
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94450.025.5983051
GTATCAA185950.021.8446751
ATCAACG225550.017.6246533
TCAACGC227650.017.4521884
CAACGCA231450.017.2920155
AACGCAG238200.016.8496466
CGCGGTA5250.016.71214543
TATCAAC240750.016.6801052
GTACATG281800.014.6633431
GTATTAG32500.014.4748951
CGAACTA7000.014.46279329
TACATGG279950.014.2319672
ACGTCCG5700.014.20870543
ACGCAGA281650.014.1944177
CGCAGAG287900.013.8706438
ACATGGG282050.013.4799253
CCGGTCG6400.013.3580649
GTATTAT30700.012.966111
CGGTCGT6600.012.95327410
AGAGTAC292100.012.91639611
Produced by FastQC (version 0.11.5)