FastQCFastQC Report
Wed 25 May 2016
SRR1700157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9259549
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA112760.12177698935444912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA750.001112007817.99763540
GTATCAA224550.016.409821
GGTATCA143900.015.9708761
GTACATG254200.014.6826421
GTACCTG49900.014.23393251
TACATGG253450.013.9535832
TCAACGC262800.013.7310724
ATCAACG264250.013.6557263
TACCTGG49600.013.5617262
CAACGCA266650.013.5496915
AACGCAG272450.013.3357736
ACATGGG256800.013.2722043
TATCAAC275150.013.2292272
GTATTAA31150.012.7805761
AGAGTAC259750.012.71467811
TCTATAC20850.012.6242413
CGAACTA4100.012.62070129
GAGTACT142200.012.35623712
ATAATAC29950.012.3189163
CCGGTCG4400.012.2713799
Produced by FastQC (version 0.11.5)