FastQCFastQC Report
Wed 25 May 2016
SRR1700156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7308963
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79670.10900315133624291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95650.020.1708221
GTATCAA171150.019.8628521
ATCAACG212450.015.5769683
TCAACGC212950.015.5720874
CAACGCA215900.015.4009965
AACGCAG220700.015.0662456
TATCAAC222500.015.0048232
CCGATCG1056.047898E-414.99818518
TACCTGG38900.014.5161392
GTACCTG42150.014.0592861
GTACATG203900.014.0324291
TACATGG204050.013.4729162
ACGCAGA251000.013.1758777
TATAACG4100.013.1690472
AGAGTAC234850.013.16230911
GTATTAG19550.013.073481
GTATATA31850.012.9957451
CGCAGAG255350.012.9073678
GAGTACT132550.012.72967812
ACATGGG208600.012.6613733
Produced by FastQC (version 0.11.5)