FastQCFastQC Report
Wed 25 May 2016
SRR1700155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8674282
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91350.021.8395561
GTATCAA170400.021.199181
TCAACGC208600.016.7919464
ATCAACG209600.016.7118323
CAACGCA210950.016.6475415
GTACCTG52400.016.576151
TACCTGG51500.016.2940222
AACGCAG217750.016.2105056
TATCAAC218250.016.1319482
CGCGGTA3050.014.75220343
GTATTAG23950.013.9778371
ACCTGGG58700.013.8738563
ACGCAGA256900.013.7314437
GTACATG230150.013.5530491
CGCAGAG261900.013.4177528
AGAGTAC249450.013.14946911
TATAACG5900.012.9644372
GAGTACT135900.012.92902612
TACATGG230000.012.882042
TATACTG29150.012.811395