FastQCFastQC Report
Wed 25 May 2016
SRR1700154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7136213
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68900.024.2354621
GTATCAA134950.022.3832191
TCAACGC171900.017.2620724
ATCAACG171600.017.213593
CAACGCA175050.017.0157035
TATCAAC179450.016.623562
AACGCAG181100.016.4846426
ACGCAGA210900.014.1767117
GTACATG185800.014.0288121
GTACCTG43100.013.9642781
CGCAGAG214250.013.9235438
TACCTGG42400.013.42392
TACATGG187300.013.3684622
AGAGTAC214150.013.2261911
GTATTAA23750.012.95655251
GAGTACT121700.012.7551512
TACGGGT4950.012.7255584
CAGAGTA230150.012.7074810
ACATGGG188600.012.6083233
GCAGAGT235100.012.6026029
Produced by FastQC (version 0.11.5)