FastQCFastQC Report
Wed 25 May 2016
SRR1700153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10119125
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101830.10063123046706113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111750.023.2168961
GTATCAA210750.020.8971271
TCAACGC259200.016.5430534
ATCAACG260400.016.4754583
CAACGCA263550.016.3297565
TATCAAC271000.015.988762
AACGCAG271400.015.9405636
GTACATG282400.014.2014371
ACGCAGA313350.013.7634957
TACATGG281250.013.7102722
CGCAGAG319650.013.4429638
AGAGTAC303300.013.37394911
ACATGGG283850.013.2201053
GAGTACT169400.012.80265312
ATAACGC5200.012.54649453
CAGAGTA339000.012.42345710
TAATACG4550.012.3610794
GTACCTG53550.012.2919781
GCAGAGT348700.012.2585189
TCTATAC26000.012.1138573
Produced by FastQC (version 0.11.5)