FastQCFastQC Report
Wed 25 May 2016
SRR1700152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6401014
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72310.11296647687382032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70200.024.5341681
GTATCAA133250.021.706381
ATCAACG168000.016.8985883
TCAACGC168550.016.8834864
CAACGCA171800.016.6033785
TATCAAC173550.016.4100322
AACGCAG176800.016.1974436
GTACATG180800.014.4454531
GTACCTG35900.014.31051351
ACGCAGA204150.014.0064167
TAGACCG1453.114262E-513.9628755
TACATGG179350.013.7219642
CGCAGAG209000.013.6813878
TACCTGG36650.013.3808242
ACATGGG180700.013.0716823
AGAGTAC204950.013.06260811
GTATATA27550.012.9795381
CGGCTAT3650.012.94427430
ACGTCCG3152.1827873E-1112.85611643
GTATTAT19450.012.6832531
Produced by FastQC (version 0.11.5)