FastQCFastQC Report
Wed 25 May 2016
SRR1700151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10286419
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA109300.10625660883539743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA247400.018.2349831
GGTATCA152200.017.1925771
TCAACGC300250.014.7224314
GTACCTG52400.014.6442181
ATCAACG300900.014.5859623
CAACGCA304750.014.4902735
AACGCAG308900.014.3248696
GTACATG291800.014.289011
TATCAAC313800.014.1870742
CCGGTCG4550.013.84469
TACATGG292150.013.8370072
TACCTGG52750.013.5614032
AGAGTAC304800.013.16781311
ACATGGG299850.012.9790243
GTATTAA34000.012.6494741
GTATATA42650.012.3661551
GAGTACT164500.012.36335412
ACGCAGA357050.012.3183217
CGCAGAG365250.012.0109738
GTATTAG26400.012.0038871
Produced by FastQC (version 0.11.5)