FastQCFastQC Report
Wed 25 May 2016
SRR1700150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8549402
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA163700.021.4180471
GGTATCA85950.020.9888951
GTACCTG52400.017.537671
TACCTGG50000.017.4115662
ATCAACG199450.016.9971493
TCAACGC199950.016.9321444
CAACGCA203400.016.6449475
TATCAAC207300.016.5488362
AACGCAG208800.016.2360236
ATAACGC4650.014.9970423
GTACATG216850.014.5923381
ACCTGGG61250.014.0666143
TATAACG5300.014.0066732
TACATGG217800.013.9332032
ACGCAGA244050.013.8468677
CGCAGAG249850.013.5434368
AGAGTAC234950.013.4639611
TAACGCA4900.013.3137024
ACATGGG218450.013.2944723
GAGTACT132750.012.84687912
Produced by FastQC (version 0.11.5)