FastQCFastQC Report
Wed 25 May 2016
SRR1700149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8556645
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80200.026.8933981
GTATCAA161750.022.4153441
TCAACGC197800.017.9809864
ATCAACG197250.017.9740983
CAACGCA200400.017.7140225
AACGCAG206300.017.283756
TATCAAC206950.017.2403342
GTACATG222600.014.9966411
ACGCAGA239250.014.8759697
CGCAGAG242250.014.6081678
TACATGG221450.014.5978532
ACATGGG226150.013.8368883
AGAGTAC249300.013.39190511
GAGTACT144200.013.26124912
CAGAGTA264650.012.938189510
GCAGAGT270600.012.9114329
TATACTG26600.012.685795
AGTACTT150800.012.57641913
GTATTAG22200.012.5394731
CATGGGG140650.012.3796714
Produced by FastQC (version 0.11.5)