FastQCFastQC Report
Wed 25 May 2016
SRR1700148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7244476
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA107240.14803003005324333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA185300.015.8615151
GTATTAG20300.015.6042511
GTACATG195500.014.3974151
GGTATCA120750.014.2221591
TACATGG194150.014.020222
ACATGGG195800.013.3850523
GTACCTG37150.013.2163581
TCAACGC215850.013.1422554
ATCAACG216250.013.1387513
CAACGCA218350.012.9711775
AACGCAG221050.012.8534496
AGAGTAC196800.012.83826811
TATCAAC225800.012.722542
GAGTACT101950.012.6670812
ATAACGC4150.012.4677073
CATGGGG133650.012.3715814
TACCTGG37650.012.129362
ATAATAC25350.012.0689033
TAATACT27850.012.0356054
GTATTAA23850.011.7637071
Produced by FastQC (version 0.11.5)