FastQCFastQC Report
Wed 25 May 2016
SRR1700147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8602700
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102500.020.6858161
GTATCAA182900.020.079911
TCAACGC222500.016.0758784
ATCAACG222650.016.0549453
CAACGCA225550.015.928315
AACGCAG231600.015.5607876
TATCAAC232000.015.49517252
GTACATG221750.014.2965571
GTATTAG23450.014.0885711
GTACCTG49850.013.754171
TACCTGG47350.013.6829632
ACGCAGA264350.013.582717
TACATGG223700.013.5559992
AGAGTAC250050.013.35180411
CGCAGAG269400.013.3030448
GAGTACT139600.012.92474112
ACATGGG228750.012.8830243
GTATATA43950.012.7687891
ATAACGC5250.012.4264293
TAGACCG1852.2509033E-512.1600655
Produced by FastQC (version 0.11.5)