FastQCFastQC Report
Wed 25 May 2016
SRR1700146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6009349
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74270.12359075833339019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG56250.027.5438541
TACCTGG56350.026.8288652
ACCTGGG61000.024.1936263
TATAACG4050.019.997442
GTATCAA129700.019.7232881
CCTGGGG49800.019.6962744
GGTATCA75700.019.3912371
ATAACGC4900.017.9058723
TAACGCA5000.017.0978134
GTACCGG4800.016.0211751
ATCAACG157950.015.7956993
TCAACGC159350.015.67104154
CAACGCA161500.015.4763475
TATCAAC167150.015.1147282
AACGCAG166950.015.0522336
GTACATG151200.014.4205531
GTATTAG14700.014.1555631
GTACACG5500.013.9821171
TACATGG153250.013.8432532
TACACGG4900.013.7737472
Produced by FastQC (version 0.11.5)