FastQCFastQC Report
Wed 25 May 2016
SRR1700145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8086703
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84650.020.6838021
GTATCAA155350.020.1239451
ATCAACG186000.016.4131583
TCAACGC186200.016.34724
CAACGCA189450.016.0548925
AACGCAG193650.015.7532486
TATCAAC199600.015.4075382
GTACATG190000.014.2156611
GTACCTG41050.014.0521691
GTATTAG23750.013.810861
TACATGG190600.013.7272062
ACGCAGA225350.013.4873237
CGCAGAG230750.013.1131968
AGAGTAC218900.013.00087911
ACATGGG193300.012.988463
TACCTGG41800.012.9707792
GAGTACT132550.012.89917212
TATACTG29500.012.8881315
ATACCGC4550.012.3610857
GTATTAT30100.012.0245771
Produced by FastQC (version 0.11.5)