FastQCFastQC Report
Wed 25 May 2016
SRR1700144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6619561
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA74640.1127567220847425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69290.10467461512931144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA600.00651827718.74771512
CGCGGTA2001.8189894E-1217.99767143
GTATCAA166000.017.8929081
GGTATCA100750.017.0420191
ACCGTCC2900.014.7395838
TCAACGC199450.014.5167984
ATCAACG200650.014.4748273
CAACGCA202450.014.3794695
GTACCTG36900.014.1615221
TATCAAC207250.014.0789972
AACGCAG207350.014.0723146
GTACATG180850.013.9845231
GAGTACT115750.013.43032812
TACATGG180700.013.25921252
TACCTGG36400.013.226312
AGAGTAC202200.013.151216511
GTATTAG19200.012.9603221
AGTACTT120700.012.76761113
ACATGGG182100.012.6631063
ACGCAGA234650.012.444687
Produced by FastQC (version 0.11.5)