FastQCFastQC Report
Wed 25 May 2016
SRR1700143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9110131
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101300.022.2390271
GTATCAA187950.020.5908161
TCAACGC229600.016.3829774
ATCAACG230150.016.3633773
CAACGCA231450.016.2423065
TATCAAC240400.015.8622092
AACGCAG238500.015.8000056
GTACATG251850.014.5940981
TACATGG252050.014.120442
GTATTAG24800.013.7718131
ACGCAGA275550.013.6429057
CGCAGAG278950.013.4604868
ACATGGG255600.013.3434093
AGAGTAC263650.013.29445411
GAGTACT145150.013.11245912
TATACTG28850.013.0226415
TATAACG4450.012.6388752
GTATATA39850.012.4303021
AGTACTT151300.012.41583713
GTACCTG47150.012.3766721
Produced by FastQC (version 0.11.5)