FastQCFastQC Report
Wed 25 May 2016
SRR1700142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7697614
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92590.12028402567340997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84150.025.616211
GTATCAA167150.021.8816341
TCAACGC211150.016.8874344
ATCAACG211850.016.8528753
CAACGCA215300.016.6141685
TATCAAC219000.016.3437462
AACGCAG220850.016.2680636
GTACCTG37650.014.118191
GTACATG239100.013.9252931
ACGCAGA257750.013.8519037
CGCAGAG263650.013.4651268
TACATGG241200.013.3657842
GTATTAG19750.013.1706381
ACATGGG241200.013.0113533
AGAGTAC261300.012.91466111
GAGTACT142600.012.85787712
TATACTG25600.012.8303475
TACCTGG38950.012.2448632
CAGAGTA282900.012.21488710
GCAGAGT290500.012.15088759
Produced by FastQC (version 0.11.5)