FastQCFastQC Report
Wed 25 May 2016
SRR1700141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6287482
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94590.15044178257687257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67610.10753112295192258No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA63430.1008829925874937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG58100.024.4069751
TACCTGG56650.024.3040262
TAACGCA4200.022.4971084
ACCTGGG61100.022.0920893
ATAACGC4600.022.008043
TATAACG5100.019.409272
GGTATCA96550.018.9269941
GTATCAA168150.018.4938951
CCTGGGG47850.016.502584
ATCAACG202300.014.9795383
TCAACGC204200.014.8732114
TATCAAC209000.014.6930882
CAACGCA207150.014.6614045
AACGCAG212700.014.3742636
GTACATG164900.014.1540791
GTATAAC11850.014.1232911
GTATTAG18200.014.0420211
TAACGGC3250.013.84448436
GTACACG4150.013.6243081
TACATGG166600.013.4091412
Produced by FastQC (version 0.11.5)