FastQCFastQC Report
Wed 25 May 2016
SRR1700140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7268886
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75680.10411499093533727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA165050.019.5085411
GGTATCA97500.018.4036481
TCAACGC201950.015.5947664
ATCAACG202450.015.5673623
CAACGCA203400.015.4946535
TATCAAC207950.015.2205322
AACGCAG208550.015.1553786
GTACATG201500.014.9127731
TACATGG204450.014.32578852
ACATGGG208150.013.67126753
AGAGTAC213950.013.5545811
GAGTACT119200.013.51394812
TATAACG3500.013.497272
GTACCTG38900.013.2623541
TACCTGG38350.013.1981132
ACGCAGA241500.013.0237337
TATACTG22850.012.9951835
CGCAGAG246000.012.8037858
AGTACTT125300.012.76609413
GTACCGG4100.012.6934411
Produced by FastQC (version 0.11.5)