FastQCFastQC Report
Wed 25 May 2016
SRR1700139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7624616
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104000.019.844741
GTATCAA171900.018.2724821
GTACCTG42650.015.9179151
TCAACGC209350.014.6891694
TACCTGG43500.014.6869222
ATCAACG208900.014.6777373
CAACGCA212600.014.4751995
AACGCAG216800.014.2364666
TATCAAC217700.014.2290942
GTACATG197750.014.0986691
TACATGG197750.013.5486782
GTATTAG21500.013.1569591
ACATGGG202450.012.7785563
AGAGTAC228550.012.54134211
GTATAGG15500.012.4099821
GTATTAA25250.012.3680621
GTATATA35350.012.3552591
ACGCAGA250750.012.2922847
ACCTGGG50850.012.0773983
CGCAGAG254750.012.0551178
Produced by FastQC (version 0.11.5)