FastQCFastQC Report
Wed 25 May 2016
SRR1700138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6276927
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83750.018.7278881
GTATCAA139150.018.1681351
GTACCTG37050.017.8375421
TACCTGG36950.017.4121072
GTACATG165950.015.0158681
TCAACGC170200.014.6711164
ATCAACG170350.014.6449923
CAACGCA172700.014.4717625
TACATGG165150.014.4386642
TATCAAC176550.014.2581142
AACGCAG176550.014.2326316
ACCTGGG43500.014.2214253
ACATGGG168450.013.7952323
CGGCTAT4150.013.55379230
TCGTGCG2205.523725E-813.2939799
GTATTAG17400.013.1374921
TACCGTC2451.6241756E-812.8557167
CGCGGTA3001.2914825E-1012.74899143
AGAGTAC184150.012.55901811
ACGCAGA201750.012.3778097
Produced by FastQC (version 0.11.5)