FastQCFastQC Report
Wed 25 May 2016
SRR1700137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8507545
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA97200.11425152614532161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA201750.017.3499721
GTACCTG47650.016.0025421
GGTATCA127000.015.1260641
TACCTGG51300.014.5145462
GTACATG223950.014.4580771
TCAACGC240900.014.296554
ATCAACG240600.014.2863253
CAACGCA243600.014.1473245
AACGCAG248500.013.9499176
GTATTAG23150.013.8790371
TACATGG225700.013.8440612
TATCAAC251950.013.687392
AGAGTAC240200.013.33730611
ACCTGGG55950.013.1072133
ACATGGG229500.013.0953353
GAGTACT136150.013.08695712
CCTATAC14100.013.0824053
CGACCGT3202.910383E-1112.65460221
GTATAAG23350.012.5004251
AGTACTT141400.012.26686413
Produced by FastQC (version 0.11.5)