FastQCFastQC Report
Wed 25 May 2016
SRR1700136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6693356
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64150.027.2796461
GTATCAA124250.022.5387171
TCAACGC158050.017.2364944
ATCAACG159200.017.0837253
CAACGCA160800.016.9277295
TATCAAC165950.016.6057382
AACGCAG165250.016.4854956
GTACCTG38600.015.073071
GTACATG212450.014.7054481
GTATTAA21700.014.6057621
TACCTGG37100.014.4918332
TACATGG210500.014.0210672
ACGCAGA195000.013.8791487
GTATTAG19500.013.8155521
ACCGCGT2652.4556357E-1013.583328
TATACTG22700.013.5766945
CGCAGAG199200.013.575228
CGCGGTA4200.013.39139243
TTAACGG4250.013.23305535
ACATGGG217050.013.0693693
Produced by FastQC (version 0.11.5)