FastQCFastQC Report
Wed 25 May 2016
SRR1700135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7818306
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100450.019.5834831
GTATCAA172150.018.9222951
TCAACGC208350.015.2993984
ATCAACG207500.015.2861823
CAACGCA211300.015.1070935
TATCAAC214200.014.9550722
AACGCAG214600.014.937686
GTACCTG38550.014.9152121
GTACATG203400.014.4347451
TACATGG202150.014.088282
ACATGGG205150.013.2681963
AGAGTAC223700.013.26542811
GAGTACT127900.013.13991412
TACCTGG41650.013.1246972
ACGCAGA245850.013.0128337
CGCAGAG250350.012.7339968
AGTACTT132450.012.56962213
GTATTAG22000.012.553281
GTATTAA27250.012.3776961
GTATAGG16150.012.0544111
Produced by FastQC (version 0.11.5)