FastQCFastQC Report
Wed 25 May 2016
SRR1700134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4595938
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51830.11277349694447576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42650.026.7958622
GTACCTG43700.026.3442331
ACCTGGG47400.023.3037743
GGTATCA51250.022.2426171
TATAACG3750.020.397212
TAACGCA4150.020.0574954
GTATCAA94900.019.8530831
ATAACGC4050.019.4417843
CCTGGGG35550.018.605054
ATCAACG115800.015.9304623
TCAACGC116650.015.7950944
CAACGCA118850.015.5784325
TATCAAC121350.015.4058042
GTACACG4000.015.26697351
AACGCAG123150.015.2357626
TATCACG3500.014.783692
GTACCGG3850.014.6868441
GTATAAC9550.014.6837671
GTATATA22750.014.0180271
GTACATG104800.013.9418511
Produced by FastQC (version 0.11.5)