FastQCFastQC Report
Wed 25 May 2016
SRR1700133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5006155
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA53980.10782726463723157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG30450.018.2712041
TACCTGG30850.017.7206022
GTATCAA123450.016.7262671
GGTATCA77400.016.2754731
ACCTGGG33950.015.7711943
CCGACGC1451.8135106E-615.51573632
CGAACTA2601.0913936E-1114.71011229
CGTCGAC950.0053838814.20872242
GTATTAG15550.014.1078451
GTATAGG10300.014.0527741
GTACATG124100.013.9961571
TACACCG2905.456968E-1213.9637435
GTACCGT1951.8353967E-713.8446726
AATACCG3101.8189894E-1213.7885725
TCAACGC147650.013.7588364
ATCAACG147550.013.75291253
CAACGCA150300.013.5461845
TATCAAC152950.013.370322
TACATGG122650.013.3166962
AACGCAG154150.013.2373126
Produced by FastQC (version 0.11.5)