FastQCFastQC Report
Wed 25 May 2016
SRR1700132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6391247
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64080.10026212412069194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA151500.020.6667731
GGTATCA81350.020.5789571
TCAACGC182350.016.7662934
ATCAACG183200.016.676223
CAACGCA184900.016.5837335
TATCAAC191400.016.1498362
AACGCAG190900.016.0979846
GTACCTG36700.015.59565451
TACCTGG35250.014.93413452
TATAACG3100.014.5141512
TAACGGC2853.6379788E-1214.20870136
ACGCAGA217050.014.1689887
GAGTACT120950.014.06196612
CGCAGAG219550.013.9871538
AGAGTAC202900.013.98180211
CGCGGTA2905.456968E-1213.963613543
ATAACGC4350.013.96361353
GTACATG168100.013.929061
GTATTAG20350.013.6738211
AGTACTT125400.013.43726913
Produced by FastQC (version 0.11.5)