FastQCFastQC Report
Wed 25 May 2016
SRR1700131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110908
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61850.025.8948061
GTATCAA129050.022.7703781
TCAACGC160100.017.9863554
ATCAACG161200.017.7938393
CAACGCA162250.017.7480145
TATCAAC168500.017.1564622
AACGCAG168000.017.1539576
ACGCAGA196000.014.6804377
CGCAGAG199750.014.3597838
GTACATG189200.014.3595551
TACATGG188100.013.8378642
GTATTAG20800.013.594581
AGAGTAC206250.013.32912811
GTATATA29100.012.9820291
CAGAGTA218350.012.86867210
GCAGAGT222200.012.8178199
ACATGGG193950.012.8173133
CGCGGTA4750.012.78776543
GTATTAA23900.012.6831171
GAGTACT112800.012.5648112
Produced by FastQC (version 0.11.5)