FastQCFastQC Report
Wed 25 May 2016
SRR1700130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6055929
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63350.024.2117161
GTATCAA120550.021.2995851
TCAACGC148450.016.8368194
ATCAACG149700.016.7262863
CAACGCA150300.016.6595135
AACGCAG155500.016.1750166
TATCAAC156650.016.1278152
ATAACGC3800.014.8007123
CCGGTCG2509.640644E-1114.3983719
GTACATG152550.014.384741
ACGCAGA179650.013.9255047
TACATGG152250.013.8011662
CGCAGAG182900.013.6903598
TACCTGG30750.013.607992
GTATTAG17150.013.454841
CTAGTAC7150.013.2150693
GTACCTG33650.013.1769081
ACATGGG153350.013.1740323
AGAGTAC181350.013.12506411
GAGTACT110300.013.09461912
Produced by FastQC (version 0.11.5)