FastQCFastQC Report
Wed 25 May 2016
SRR1700129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6317903
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54950.027.3291131
GTATCAA115850.022.7629051
TACCTGG45000.022.6972582
GTACCTG46700.022.374341
ATAACGC4050.020.5530723
ACCTGGG48150.020.3246463
TAACGCA4600.019.0737824
TCAACGC141750.018.0454394
ATCAACG141800.018.023213
CAACGCA145750.017.6428075
TATCAAC150250.017.1593252
AACGCAG152200.017.0726456
TATAACG4600.016.1393532
CCTGGGG37600.015.2574664
GTATTAG18400.015.2415471
ACGCAGA173950.014.8862187
CGCAGAG177950.014.538968
CGACCGT1951.8359606E-713.844480521
GTACATG140850.013.7288581
GTACCGG5000.013.5698921
Produced by FastQC (version 0.11.5)