FastQCFastQC Report
Wed 25 May 2016
SRR1700128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5521414
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128400.018.700351
GGTATCA75700.017.7877251
ACGCGCG952.8253038E-416.57624438
TAGACCG1401.2590699E-616.0686635
TCAACGC155200.015.2486654
ATCAACG155700.015.1563523
CAACGCA157300.015.0164885
GTATTAG16200.014.807791
GTACATG140400.014.7809261
AACGCAG161050.014.7366756
TATCAAC162700.014.6701862
TACATGG141450.014.234032
GTACCTG29450.014.2162741
TACCTGG28300.013.9905262
ATAATAC20500.013.826893
AGAGTAC162400.013.79738611
ACATGGG143050.013.6187683
GAGTACT96750.013.20750812
ACGCAGA182500.012.9927557
CGCAGAG185900.012.7672278
Produced by FastQC (version 0.11.5)