FastQCFastQC Report
Wed 25 May 2016
SRR1700127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7408780
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82650.021.028611
GTATCAA147800.019.8130841
TCAACGC180800.015.8888274
ATCAACG180400.015.8617083
CAACGCA183350.015.6310425
AACGCAG188050.015.3002846
TATCAAC190450.015.1900572
CGCGGTA3750.014.99824343
GTACATG212700.014.8103041
TACATGG213400.014.2521922
ACATGGG216050.013.70253853
ACGCAGA218200.013.1667787
CGCAGAG222550.012.9296368
GTATTAG21100.012.7631161
GTACCTG33600.012.6622481
AGAGTAC213600.012.58673111
GTATATA32750.012.5762831
ACGTCCG4150.012.46841943
GAGTACT113850.012.35074812
CATGGGG142050.012.3049314
Produced by FastQC (version 0.11.5)