FastQCFastQC Report
Wed 25 May 2016
SRR1700126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7350806
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144400.020.5010091
GGTATCA80200.019.2744061
CGCGGTA4950.016.81649643
TCAACGC176750.016.278624
ATCAACG176200.016.2655963
CAACGCA178850.016.0749055
AACGCAG182650.015.7651016
TATCAAC186100.015.6299882
TTAACGG5650.015.13050735
TAACGGC5550.014.99768136
GTACATG204750.014.9226021
CGGTACC5900.014.49007245
TACATGG202900.014.33583452
GTACCTG36900.014.1070481
ACATGGG203750.013.7460593
GTATTAG23500.013.6758761
GCGGTAC6450.013.60328844
ACGCAGA212550.013.5483037
ATAATAC27650.013.3430543
GTATATA30700.013.2699361
Produced by FastQC (version 0.11.5)