FastQCFastQC Report
Wed 25 May 2016
SRR1700125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4706971
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG46850.026.8092461
TACCTGG47800.025.414172
ACCTGGG49250.024.0721233
TAACGCA4150.021.141054
ATAACGC3950.020.5029073
TATAACG3950.019.9333822
CCTGGGG37000.019.2130094
GTATCAA97150.019.194891
GGTATCA55450.018.529151
TCAACGC115750.015.8396884
ATCAACG115650.015.7950293
CAACGCA118400.015.4851695
TATACTG16400.015.363295
AACGCAG122200.015.15090756
TATCAAC122250.015.1263092
CGACGCC1654.2117244E-714.99877133
GTACATG96300.014.100231
GTACACG3550.014.0247631
TCTGTCG6600.013.9756828
CTGTCGC6800.013.5646339
Produced by FastQC (version 0.11.5)