FastQCFastQC Report
Wed 25 May 2016
SRR1700124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6563201
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131800.019.3988691
TACCTGG41750.017.511842
GGTATCA77700.017.26821
GTACCTG44000.017.0211431
TCAACGC158200.015.7841584
ATCAACG159000.015.7047393
CAACGCA161500.015.48949055
GTACATG172450.015.0492061
AACGCAG166400.015.0468886
TATCAAC167650.014.9346982
ACCTGGG49500.014.4974083
TACATGG173450.014.357482
GTATTAA23800.014.1651991
GTATTAG21550.013.8592591
ACATGGG174250.013.8267973
TAACGGC3900.013.26716236
TATACTG23550.013.1823565
CGCGGTA3950.013.09982143
AGAGTAC178700.012.95484211
ACGCAGA192700.012.9359797
Produced by FastQC (version 0.11.5)