FastQCFastQC Report
Wed 25 May 2016
SRR1700123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6679333
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51750.025.804111
GTATCAA107800.023.4730911
TCAACGC132050.018.5859894
ATCAACG132450.018.4789073
CAACGCA135200.018.2028755
TATCAAC139950.017.7297232
AACGCAG139600.017.6937376
ACGCAGA165700.014.8946047
GTACATG171250.014.60423851
GTACCTG34000.014.5784871
CGCAGAG169500.014.5208668
TACATGG169800.014.0564962
TACCTGG35450.013.7702832
GTACCGG3650.013.641971
ACGTCGA1000.007559613.49903132
ACATGGG173150.013.3168253
TAGACCG1709.437539E-613.232765
AGAGTAC177650.013.19615511
GTATTAG20150.013.02957251
CAGAGTA186350.012.9301910
Produced by FastQC (version 0.11.5)