FastQCFastQC Report
Wed 25 May 2016
SRR1700122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7923448
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA143090.1805905711755791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99110.1250844329387913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG66750.024.8600061
TACCTGG65550.024.6067352
ACCTGGG69300.022.9505833
TATAACG5950.017.770042
CGCGGTA3600.016.87208743
TAACGCA5900.016.7767644
CCTGGGG56900.016.4470734
ATAACGC6200.016.3278263
GTATCAA227500.015.03597451
TTAACGG4800.014.99769535
TAACGGC4450.014.6603936
GTATTAG25600.014.3259551
CCGACGC2052.1875167E-814.26645932
GTACACG5800.014.0515121
GTACCCG6650.013.95761
GTACATG180900.013.8409241
TAGACCG1656.934064E-613.6340095
TACACGG5850.013.4592152
GTACCGG6650.013.2767411
GGTATCA154150.013.276191
Produced by FastQC (version 0.11.5)