FastQCFastQC Report
Wed 25 May 2016
SRR1700121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9301080
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105060.1129546246242372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135250.018.0919951
GTATCAA224950.017.8410661
GTACCTG50400.017.2014981
TACCTGG51200.016.0374322
ATCAACG266100.014.6763063
TCAACGC266950.014.6632824
GTACATG219600.014.5649131
CAACGCA269750.014.502745
AACGCAG274500.014.2764446
ACCTGGG56150.014.2229773
TATCAAC279200.014.13273052
TACATGG220300.013.7142932
ACATGGG220550.013.3315423
TATACTG34150.013.2408925
AGAGTAC279700.013.02333711
GTATTAG28650.012.7203571
ATAACGC5700.012.6295253
ACGCAGA310950.012.6261367
GTATAAA42550.012.6080181
GAGTACT179350.012.51979112
Produced by FastQC (version 0.11.5)