FastQCFastQC Report
Wed 25 May 2016
SRR1700120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7686193
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103730.13495627809502053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113250.020.3905891
GTATCAA199200.019.1390571
TATAACG4200.014.9973052
TCAACGC249750.014.9882984
ATCAACG249200.014.9852693
CAACGCA252550.014.8221245
TATCAAC254850.014.6971822
CCGATCG2152.590241E-914.6504369
AACGCAG257050.014.5801466
GTACATG211450.014.5398471
TACATGG211500.014.0506662
GTACCTG42400.014.0429281
TACCTGG41050.013.9743462
TAACGCA4100.013.7170474
CGATCGA1000.00755667913.49985810
ACATGGG212300.013.4361153
ATAACGC5050.013.3639353
GTATTAG22150.013.1851111
AGAGTAC269600.012.98576811
AGGACCG6250.012.9576715
Produced by FastQC (version 0.11.5)